Nitrotetrazolium blue chloride CAS:298-83-9

Nitrotetrazolium blue chloride (NBT) is commonly used in biochemical and biological research as a substrate to detect the activity of certain enzymes. When NBT is reduced by these enzymes, it forms an insoluble blue or purple formazan product. This reaction is often used to study the activity of nitroreductases and other enzymes involved in redox processes. NBT is frequently employed in assays to assess redox reactions, cellular oxidative stress, and enzyme activity. Its applications span across various fields including immunology and microbiology, where it is used to investigate enzyme function and cellular processes.