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N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt CAS:66992-27-6

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, also known as HEPES sodium salt, is a chemical compound commonly used as a pH buffering agent in biological and chemical laboratories. It helps maintain a stable pH range, making it ideal for applications such as cell culture, enzyme assays, protein studies, electrophoresis, and pharmaceutical formulations. HEPES sodium salt ensures optimal conditions for biological processes and enhances the accuracy and reliability of experimental results.


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Application and Effect

pH buffering agent: HEPES sodium salt is a zwitterionic buffering compound that helps maintain a stable pH range in biological experiments. It is commonly used in cell culture media and biochemical assays, providing a suitable environment for cell growth and enzyme function.

Protein studies: HEPES sodium salt is widely used as a buffer in protein purification, characterization, and analysis. It helps maintain the protein's stability and activity, ensuring accurate and reliable results.

Electrophoresis: HEPES sodium salt is commonly used as a running buffer in electrophoresis techniques such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). It helps maintain the pH and ionic strength, allowing efficient separation of biomolecules.

Pharmaceutical formulations: HEPES sodium salt is used as a buffering agent in the formulation of pharmaceutical products, including injections and topical preparations. It ensures the stability and efficacy of drugs and helps to maintain their pH within a desired range.

Cell culture applications: HEPES sodium salt is frequently used in cell culture media to maintain a stable pH and provide an optimal environment for cell growth and viability. It helps to minimize the pH changes resulting from cellular metabolism and carbon dioxide buildup.

Product Packing:

6892-68-8-3

Additional Information:

Composition C6H14NNaO5S
Assay 99%
Appearance White powder
CAS No. 66992-27-6
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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