N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt CAS:66992-27-6

pH buffering agent: HEPES sodium salt is a zwitterionic buffering compound that helps maintain a stable pH range in biological experiments. It is commonly used in cell culture media and biochemical assays, providing a suitable environment for cell growth and enzyme function.

Protein studies: HEPES sodium salt is widely used as a buffer in protein purification, characterization, and analysis. It helps maintain the protein's stability and activity, ensuring accurate and reliable results.

Electrophoresis: HEPES sodium salt is commonly used as a running buffer in electrophoresis techniques such as SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). It helps maintain the pH and ionic strength, allowing efficient separation of biomolecules.

Pharmaceutical formulations: HEPES sodium salt is used as a buffering agent in the formulation of pharmaceutical products, including injections and topical preparations. It ensures the stability and efficacy of drugs and helps to maintain their pH within a desired range.

Cell culture applications: HEPES sodium salt is frequently used in cell culture media to maintain a stable pH and provide an optimal environment for cell growth and viability. It helps to minimize the pH changes resulting from cellular metabolism and carbon dioxide buildup.