p-Nitrophenyl -a-L-Fucopyranoside CAS:10231-84-2

Enzyme activity assays: pNP-α-L-Fucopyranoside is commonly used as a synthetic substrate to measure the activity of α-L-fucosidase enzymes. The enzyme cleaves the glycosidic bond between the fucose sugar and the p-nitrophenyl group, resulting in the release of p-nitrophenol. The amount of p-nitrophenol produced can be quantified colorimetrically or spectrophotometrically, allowing the determination of enzyme activity.

Enzyme kinetics studies: The hydrolysis of pNP-α-L-Fucopyranoside can be used to determine kinetic parameters, such as the Michaelis-Menten constant (Km) and the maximum velocity (Vmax) of α-L-fucosidase activity. These parameters provide valuable information about the enzyme's efficiency and affinity for the substrate.

Inhibitor screening: pNP-α-L-Fucopyranoside can be used to screen and evaluate potential inhibitors of α-L-fucosidase. By measuring the enzyme activity in the presence and absence of different compounds, researchers can identify and characterize inhibitors that can modulate the enzyme's function.