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P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

P-Nitrophenyl beta-D-lactopyranoside, also known as PNPG, is a compound often used in enzymatic assays to measure the activity of beta-galactosidase, an enzyme involved in carbohydrate metabolism. PNPG is a synthetic substrate that can be cleaved by beta-galactosidase, resulting in the release of a yellow-colored product. The extent of substrate hydrolysis can be quantified spectrophotometrically by measuring the absorbance of the product at a specific wavelength. This allows researchers to assess the activity and kinetics of beta-galactosidase in various contexts, such as studying enzyme function, screening for enzyme inhibitors or activators, or evaluating the effects of mutations on enzyme activity.


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Application and Effect

Detection of beta-galactosidase activity: PNPG is commonly used in assays to measure the activity of beta-galactosidase, an enzyme that catalyzes the hydrolysis of lactose into glucose and galactose. The hydrolysis of PNPG by beta-galactosidase releases a p-nitrophenol (pNP) molecule, which can be detected spectrophotometrically due to its yellow color.

Screening for enzyme inhibitors and activators: PNPG can be used in high-throughput screening to identify compounds that modulate beta-galactosidase activity. By measuring the rate of PNPG hydrolysis in the presence of different test compounds, researchers can identify inhibitors that decrease enzyme activity or activators that enhance enzyme activity.

Study of enzyme kinetics: The hydrolysis of PNPG by beta-galactosidase follows Michaelis-Menten kinetics, allowing researchers to determine important enzyme parameters such as the maximum reaction velocity (Vmax) and the Michaelis constant (Km). This information helps in understanding the enzyme's substrate affinity and catalytic efficiency.

Molecular biology applications: Beta-galactosidase, which cleaves PNPG, is commonly used as a reporter gene in molecular biology. The PNPG substrate is often used to detect and visualize the expression of the reporter gene, providing a simple and sensitive way to assess gene expression in various experimental systems.

Product Sample

1
5

Product Packing:

6892-68-8-3

Additional Information:

Composition C18H25NO13
Assay 99%
Appearance White powder
CAS No. 4419-94-7
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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