PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) SALT CAS:14815-59-9

Enzyme detection: Phenolphthalein monophosphate dicyclohexylammonium salt is used to detect the presence of phosphatase enzymes in biological samples. Phosphatases catalyze the removal of phosphate groups from molecules, and their activity can be crucial in understanding various cellular processes.

Enzyme kinetics: This salt form of phenolphthalein monophosphate is used to study the kinetics of enzyme-catalyzed reactions, specifically those involving phosphatases. By measuring the rate at which the phosphate groups are hydrolyzed from the phenolphthalein monophosphate molecule, researchers can determine enzymatic activity and other kinetic parameters like Km and Vmax.

Signal generation: Phenolphthalein monophosphate dicyclohexylammonium salt is often used as a substrate or a component in reaction mixtures to generate a detectable signal. When phosphatase enzymes act upon the salt, the phosphate group is cleaved, resulting in a color change of the phenolphthalein molecule. This color change can be measured spectrophotometrically or visually, providing a quantitative or qualitative measure of phosphatase activity.

Diagnostic applications: Phenolphthalein monophosphate assays can be utilized for diagnostic purposes, particularly in the identification and quantification of phosphatase activity in biological samples. Abnormal phosphatase activity is associated with various diseases and conditions, such as liver dysfunction or bone disorders. By measuring this activity, clinicians and researchers can gain insights into the pathological conditions and monitor therapy effectiveness.