Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside is commonly used as a substrate for the enzyme β-galactosidase. When β-galactosidase acts upon it, the compound is hydrolyzed, resulting in the production of p-nitrophenol or o-nitrophenol, which have a yellow color that can be easily detected. This makes it useful as a substrate for assays that measure β-galactosidase activity.

One common application of Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside is in reporter gene assays. In these assays, a gene of interest is coupled to a reporter gene, such as the beta-galactosidase gene. The activity of the reporter gene is then monitored by measuring β-galactosidase activity using Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside as the substrate. This allows researchers to study gene expression and regulation.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside can also be used to screen for inhibitors or activators of β-galactosidase. By testing different compounds or drug candidates, researchers can identify molecules that modulate the activity of this enzyme, which could have therapeutic applications in certain diseases or conditions.

In addition, this compound can be used in protein purification. β-galactosidase is often fused to proteins of interest to facilitate their detection and purification. Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside can be used as a substrate to screen and purify these fusion proteins by detecting the β-galactosidase activity.