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Phenylgalactoside CAS:2818-58-8

Phenylgalactoside, also known as p-nitrophenyl β-D-galactopyranoside (pNPG), is a synthetic substrate frequently used in biochemical and molecular biology experiments. It is commonly used to detect and measure the activity of the enzyme β-galactosidase.

When phenylgalactoside is hydrolyzed by β-galactosidase, it releases p-nitrophenol, which is a yellow-colored compound. The liberation of p-nitrophenol can be quantitatively measured using a spectrophotometer, as the absorbance of p-nitrophenol can be detected at a wavelength of 405 nm.

 


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Application and Effect

Effect on enzyme activity: Phenylgalactoside is commonly used to measure the activity of the enzyme β-galactosidase. When phenylgalactoside is hydrolyzed by β-galactosidase, it releases p-nitrophenol. The accumulation of p-nitrophenol can be quantitatively measured, providing insights into the activity of β-galactosidase. This effect is harnessed in applications such as enzyme assays and screening systems.

Gene expression analysis: Phenylgalactoside is often used as a substrate in molecular biology experiments to study gene expression. The lacZ gene, which encodes β-galactosidase, is commonly fused with regulatory sequences of other genes of interest. The expression of the lacZ gene and the hydrolysis of phenylgalactoside by β-galactosidase can indicate the expression pattern and level of the target gene being studied.

Screening systems: Phenylgalactoside is an important component of screening systems that make use of β-galactosidase activity. One widely known example is the blue-white screening method, which is used to identify recombinant or transformed cells in molecular biology experiments. Colonies that have successfully taken up the recombinant DNA or undergone genetic recombination will express β-galactosidase, leading to the hydrolysis of phenylgalactoside and the formation of a blue color.

Protein purification: In some cases, phenylgalactoside can be used as a ligand for affinity chromatography to purify proteins that specifically bind to or are activated by β-galactosidase. The protein of interest may have an affinity tag or a fusion tag containing a β-galactosidase-binding domain. By passing the protein mixture through a column with immobilized phenylgalactoside, the desired protein can be selectively retained and subsequently eluted.

 

Product Packing:

6892-68-8-3

Additional Information:

Composition C12H16O6
Assay 99%
Appearance White powder
CAS No. 2818-58-8
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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