piperazine-1,4-bis(2-ethanesulfonic acid) disodium salt CAS:76836-02-7

Effects:

Buffering properties: PIPES can be used to maintain constant pH levels within a specific range, as it is effective at buffering in the physiological pH range of 6.1-7.5. This makes it useful in various biological experiments where pH control is critical.

Stability: PIPES is stable over a wide range of temperatures, making it suitable for use in experiments performed under varying conditions.

Applications:

Cell culture: PIPES can be used as a buffer in cell culture techniques, such as maintaining the pH of media or buffers used for cell growth and maintenance.

Protein and enzyme studies: PIPES is commonly used in protein and enzyme studies to maintain a stable pH during various reactions, especially those involving sensitive enzymes or proteins that may be affected by pH changes.

Electrophoresis: PIPES can be used as a buffer in gel electrophoresis applications, helping to maintain optimal pH conditions for DNA or protein separation.

Molecular biology techniques: PIPES can be used as a buffer in various molecular biology techniques, including DNA/RNA extraction, PCR, and DNA sequencing, ensuring accurate results by maintaining stable pH conditions.