PIPES monosodium salt CAS:10010-67-0

Buffering Agent: HEPES-Na is primarily used as a buffering agent to maintain a stable pH range in biological and biochemical experiments. It can effectively resist pH changes caused by the addition of acids or bases.

Cell Culture: HEPES-Na is often added to cell culture media to provide a stable and optimal pH environment for cell growth and viability. It helps to counteract the pH fluctuations that can occur due to the metabolic processes of living cells.

Enzyme Assays: HEPES-Na is commonly used as a buffer in enzyme assays. It helps to maintain the pH at the optimum level for enzyme activity and stability, ensuring accurate and reliable results.

Molecular Biology Techniques: HEPES-Na is widely used in various molecular biology techniques, such as DNA and RNA isolation, PCR amplification, and protein analysis. It helps maintain stable pH conditions during these procedures, which is crucial for maintaining the integrity and functionality of biological molecules.

Electrophoresis: In gel electrophoresis, HEPES-Na is used as a buffer to provide a stable pH environment for the separation of DNA, RNA, and proteins. It helps to ensure proper migration and resolution of the molecules within the gel matrix.