PIPES sesquisodium salt CAS:100037-69-2

Buffering Agent: PIPES-Na3 is commonly used as a buffering agent in various biological and biochemical experiments. It helps to maintain a stable pH in the desired range, usually between 6.1 to 7.5.

Cell Culture: PIPES-Na3 is often utilized as a buffering agent in cell culture media to maintain the pH of the medium during cell growth and experiments. It provides a stable microenvironment for cell culture and helps support cell viability and functionality.

Enzyme and Protein Studies: PIPES-Na3 is widely used in enzyme and protein studies due to its buffering capacity in the physiological pH range. It helps maintain the optimal pH for enzyme activity, stability, and protein structure.

Gel Electrophoresis: PIPES-Na3 is suitable for use as a buffering agent in gel electrophoresis techniques such as SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). It ensures the maintenance of the desired pH throughout the gel and the separation process.

Molecular Biology Techniques: PIPES-Na3 is utilized in various molecular biology techniques such as RNA and DNA purification, PCR (Polymerase Chain Reaction), and DNA sequencing. It helps maintain the desired pH and stability during these procedures.