PMSF CAS:329-98-6 Manufacturer Price

Effect:

PMSF is a serine protease inhibitor that irreversibly binds to the active site of serine proteases, thereby blocking their activity.

It modifies the serine residues via covalent bonds, leading to enzyme inactivation.

PMSF can protect proteins from degradation by inhibiting protease activity, particularly serine proteases.

Applications:

Biochemical research: PMSF is commonly used during protein purification and isolation protocols to prevent protein degradation. It helps maintain the integrity and stability of the target protein.

Enzyme inhibition: PMSF is an essential reagent for inactivating serine proteases during enzyme assays or biochemical studies. By blocking the activity of these proteases, researchers can investigate the role of serine proteases in various biological processes.

Proteolysis studies: PMSF can be used to control or adjust protease activity in cell lysates or extracts. This is beneficial for studying proteolytic events involved in cellular processes or when proteolysis needs to be regulated in the experimental setup.

General protease inhibition: Though PMSF specifically targets serine proteases, it can also have some inhibitory effect on some cysteine proteases. It is used as a general protease inhibitor when working with protein samples to minimize proteolytic degradation.