N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt is a chemical compound commonly used in various laboratory applications. It is a water-soluble derivative of tetramethylbenzidine (TMB) that contains a sulfopropyl group.
This compound is often employed as a substrate in colorimetric assays to detect the presence of enzymes such as peroxidases or horseradish peroxidase (HRP). When these enzymes are present, they can catalyze a reaction with N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt, leading to the generation of a colored product. The intensity of the color is directly proportional to the enzymatic activity, allowing for the quantification of the enzyme or the detection of specific substrates.
The water solubility of N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt makes it convenient for use in aqueous solutions and compatible with various assay systems. Its sulfopropyl group enhances the solubility and stability of the compound, enabling efficient and reliable detection in enzymatic assays.