Puromycin dihydrochloride CAS:58-58-2 Manufacturer Price

Puromycin dihydrochloride is a potent antibiotic that is commonly used in molecular biology and cell biology research. It inhibits protein synthesis by causing premature termination of protein synthesis during translation. Puromycin acts by mimicking the 3' end of the aminoacylated tRNA molecule and gets incorporated into the growing protein chain. This leads to truncation of the protein and ultimately halts the translation process.

The main application of puromycin dihydrochloride is in the selection and isolation of cells that have been genetically modified or transfected with a puromycin resistance gene. This is achieved by introducing a gene of interest along with a puromycin resistance gene into cells, and then subjecting them to puromycin treatment. Only the cells that have successfully integrated the puromycin resistance gene into their genome will survive and proliferate, allowing for the selection and isolation of the desired cells.

Puromycin dihydrochloride is also used in studies involving gene expression and protein synthesis. By inhibiting protein synthesis, it can help researchers study the dynamics of protein synthesis, identify specific proteins that are rapidly synthesized in response to certain stimuli, and analyze the mechanisms of translation.

Furthermore, puromycin dihydrochloride is used to generate stable cell lines with specific genetic modifications. By incorporating the puromycin resistance gene along with the targeted genetic modification, researchers can generate cell lines that stably express the gene of interest and puromycin resistance. This allows for long-term studies and characterization of gene function.