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TAPSO CAS:68399-81-5 Manufacturer Price

TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) is a zwitterionic buffer commonly used in biological and biochemical research. It is an efficient buffering agent with a pKa close to physiological pH, making it suitable for maintaining a stable pH in biological experiments. TAPSO is often utilized in protein purification, enzyme assays, cell culture, and electrophoresis applications. Its high water solubility and low interference with biological processes make it a popular choice in the scientific community. TAPSO is known for its minimal effect on enzyme activity and is often used as an alternative to other buffering agents like Tris or phosphate buffers.


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Application and Effect

Protein purification: TAPSO is frequently used as a buffer in protein purification techniques such as ion exchange chromatography and size exclusion chromatography. Its buffering capacity helps maintain the desired pH throughout the purification process, ensuring protein stability.

Enzyme assays: TAPSO is utilized in enzyme activity assays to provide a consistent pH environment that mimics physiological conditions. By maintaining a stable pH, TAPSO helps ensure accurate and reliable enzyme activity measurements. 

Cell culture: TAPSO is often employed as a buffer for maintaining cell culture media at a steady pH. Its zwitterionic nature reduces the interaction with cells and minimizes the potential cytotoxic effects that can arise from using other buffering agents.

Electrophoresis: TAPSO can be used as a running buffer in electrophoretic techniques, such as protein gel electrophoresis (SDS-PAGE) or capillary electrophoresis. Its buffering capacity helps maintain the desired pH during the separation process.

Product Packing:

6892-68-8-3

Additional Information:

Composition C6H14NNaO4
Assay 99%
Appearance White powder
CAS No. 68399-81-5
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


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