TMB CAS:54827-17-7 Manufacturer Price

Tetramethylbenzidine (TMB) is a chemical compound commonly used in biochemical and clinical laboratory assays.It can be measured spectrophotometrically to determine the presence or quantity of the enzyme or its substrate.

TMB is often employed as a substrate in enzyme-linked immunosorbent assays (ELISAs), where it reacts with horseradish peroxidase (HRP) to produce a blue color in the presence of its substrate. The intensity of the color is directly proportional to the concentration of the target molecule being detected, allowing for quantitative measurements.

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Application and Effect

Immunoassays: TMB is commonly used as a substrate in ELISAs to detect antigens or antibodies. For example, in sandwich ELISAs, TMB can be used to visualize the binding of a target antigen or antibody to immobilized capture antibodies.

Enzyme activity assays: TMB can be used to measure the activity of peroxidase enzymes, such as HRP. The enzymatic reaction converts TMB into a colored product, allowing the measurement of enzyme activity.

Clinical diagnostics: TMB-based assays are commonly used in clinical laboratories for various diagnostic tests. They can be used to measure the presence or quantity of specific biomarkers, enzymes, or antibodies in patient samples.

Research applications: TMB is also widely used in research laboratories for various applications, such as studying enzyme kinetics, detecting gene expression, or quantifying protein concentrations.

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Additional Information:

Composition	C16H20N2
Assay	99%
Appearance	White powder
CAS No.	54827-17-7
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.