Tricine CAS:5704-04-1 Manufacturer Price

Tricine is a zwitterionic organic compound with the chemical formula C6H13NO5S. It is widely used as a buffering agent, primarily in biochemical and biological applications. Tricine’s distinguishing feature is its unique buffering capacity at a slightly acidic pH range, making it particularly useful in experiments that require a stable and precise pH environment. It is commonly used in protein electrophoresis, molecular biology techniques, enzymatic assays, and cell culture media. Tricine helps maintain optimal conditions for various biological processes, ensuring accurate and reliable results in research and analysis.

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Application and Effect

In biochemistry and molecular biology, the "tricine effect" refers to the ability of tricine to improve the separation and resolution of proteins on SDS-PAGE gels compared to traditional glycine-based systems. Tricine is a smaller amino acid than glycine and can penetrate the polyacrylamide gel matrix more easily, resulting in better protein separation.

The tricine buffer system is especially useful for separating low molecular weight proteins (less than 20 kDa) and resolving closely migrating bands. It is commonly used in Western blotting, protein purification, and protein expression studies. Tricine is also used in combination with other buffering agents, such as Bis-Tris or MOPS, to optimize the pH range and improve protein resolution in specific applications.

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Additional Information:

Composition	C6H13NO5
Assay	99%
Appearance	White powder
CAS No.	5704-04-1
Packing	Small and bulk
Shelf Life	2 years
Storage	Store in cool and dry area
Certification	ISO.