The Belt and Road: Cooperation, Harmony and Win-Win
products

Products

TRIS-Acetate CAS:6850-28-8 Manufacturer Price

TRIS-Acetate, is a buffer commonly used in biological and biochemical experiments. It is a combination of Tris base and acetic acid, resulting in a pH-stable solution that is used to control and maintain the desired pH range for a variety of applications.TRIS-Acetate is particularly useful in DNA and RNA studies, as it provides a suitable environment for enzyme activities, electrophoresis, and gel electrophoresis. It helps maintain the stability and integrity of nucleic acids during various procedures, such as DNA sequencing, polymerase chain reaction (PCR), and agarose gel electrophoresis.In addition to nucleic acid research, TRIS-Acetate is also utilized in protein isolation and purification procedures, membrane protein extraction, and cell culture experiments. Its versatile buffering capacity makes it a valuable tool in many areas of scientific research, ensuring optimal conditions for biological reactions and maintaining the stability of enzymes and proteins.


Product Detail

Product Tags

Application and Effect

Tris-acetate (TRIS-Acetate) is a commonly used buffer in biological and biochemical experiments. It consists of a combination of tris(hydroxymethyl)aminomethane (Tris) and acetic acid, which acts as a pH regulator and stabilizer. The pH of a TRIS-Acetate buffer typically ranges from 7.4 to 8.4.
The main effect of TRIS-Acetate is to maintain a stable pH, which is crucial for numerous biological and biochemical reactions. It works as a buffer by minimizing any significant changes in pH that may occur due to added acids or bases during experimental procedures.
TRIS-Acetate finds various applications in molecular biology, biochemistry, and biotechnology:
DNA and RNA Electrophoresis: TRIS-Acetate is commonly used as a running buffer in agarose and polyacrylamide gel electrophoresis. It provides a stable pH environment during the separation of DNA and RNA fragments based on their size.
Protein Analysis: TRIS-Acetate buffers are used for protein electrophoresis, such as SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). It ensures protein stability and separation during the process.
Enzyme Reactions: TRIS-Acetate buffers are frequently used in enzyme assays and studies. It provides the optimal pH range for various enzymatic reactions and helps maintain enzyme activity.
Cell and Tissue Culture: TRIS-Acetate buffers are used in cell culture media to maintain the appropriate pH for cell growth and proliferation. It helps to maintain the physiological conditions required for cell viability.

Product Packing:

6892-68-8-3

Additional Information:

Composition C6H15NO5
Assay 99%
Appearance White powder
CAS No. 6850-28-8
Packing Small and bulk
Shelf Life 2 years
Storage Store in cool and dry area
Certification ISO.

 


  • Previous:
  • Next:

  • Write your message here and send it to us