Tris(hydroxymethyl)nitromethane CAS:126-11-4

Effect:

Buffering Capacity: Tris acts as an effective buffering agent due to its ability to accept or donate protons, maintaining a stable pH range in solutions. It is commonly used as a primary component in buffer systems to stabilize the pH of biological samples and reactions.

Applications:

Molecular Biology: Tris is widely used as a buffering agent in various molecular biology techniques, including DNA and RNA isolation, PCR, gel electrophoresis, and protein purification. It helps maintain a stable pH environment, allowing optimal conditions for enzymatic reactions and molecular interactions.

Cell Culture: Tris is often utilized in cell culture media to maintain a constant pH and osmotic balance, supporting healthy cell growth and viability.

Protein Chemistry: Tris is used in protein chemistry experiments, such as protein solubilization, protein stability assays, and protein-ligand binding studies. It helps maintain the desired pH range, ensuring proper protein folding and activity.

Enzymology: Tris is employed in various enzymatic assays to optimize the pH conditions required for enzymatic activity. It ensures reliable and reproducible results, enabling accurate measurement of enzyme kinetics and inhibition studies.

Biochemical Assays: Tris is used as a component in many biochemical assays due to its buffering properties. It maintains a constant pH during colorimetric, spectrophotometric, and enzymatic assays, enhancing the accuracy and reliability of the results.