Xylene Cyanole FF CAS:2650-17-1

Tracking Dye: Xylene Cyanole FF is often used as a tracking dye in gel electrophoresis. It is typically added to DNA or RNA samples before loading them onto the gel. The dye migrates through the gel at a known rate, allowing researchers to track the movement of the samples and estimate their size and progress during electrophoresis.

Visualization of Nucleic Acids: Along with other dyes, such as bromophenol blue, Xylene Cyanole FF helps visualize the migration of nucleic acids on the gel. The dye forms dark blue bands that are easily visible under appropriate lighting conditions, aiding in the analysis and interpretation of the results.

Marker or Reference Point: Xylene Cyanole FF can serve as a marker or reference point in nucleic acid analysis. By loading a known amount of dye along with the samples, researchers can easily identify its migration position and compare it to the migrating nucleic acids. This can help determine the efficiency of the electrophoresis process and ensure accurate size estimation.

Compatibility with Enzymes: Xylene Cyanole FF is considered relatively inert and does not inhibit enzymatic reactions or interfere with subsequent analysis of nucleic acids. This makes it suitable for various downstream applications, such as DNA sequencing, PCR, and molecular cloning, where the integrity of the nucleic acids is crucial.